FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26314259
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACG157100.0122.416444
ACGACGC157400.0121.860745
CGACGCT157000.0121.561056
CTACACG174350.0111.7732851
GACGCTC177200.0107.9081657
TACACGA182500.0106.177622
ACACGAC183300.0105.195673
ACGCTCT189100.0100.888518
CGCTCTT210300.090.7187969
TCCGATC193850.019.33960215-19
TTCCGAT204700.018.8743910-14
CTTCCGA208700.018.76271410-14
TCTTCCG219800.017.7030610-14
CCGATCT226000.016.82575815-19
CGCGACA14700.016.7664184
ACGCGAC10800.015.4377083
CGATACG8851.8189894E-1214.743754
CGATCTA71850.014.14412415-19
CGGCGAT12550.014.0102491
CGCCGTA286400.013.81990660-64
Produced by FastQC (version 0.11.5)