FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27269007
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC104050.0118.923865
CGACGCT104750.0117.5677646
CACGACG106150.0116.707734
ACACGAC126800.098.4444963
GACGCTC126600.097.390977
CTACACG131850.095.6571351
TACACGA134600.093.3350142
ACGCTCT140150.088.079418
CGCTCTT164750.074.7522059
TCCGATC129600.018.26704615-19
CTTCCGA141350.017.70284810-14
TTCCGAT141600.017.32348610-14
CGACGTA11800.016.5858549
TCTTCCG158500.015.86968710-14
CGCCGTA281550.015.40713560-64
CCGATCT157400.015.38150815-19
TCGCCGT288350.014.9633560-64
GCCGTAT290750.014.86476660-64
CGTATCA294550.014.74696865-69
GTCGCCG300050.014.45235560-64
Produced by FastQC (version 0.11.5)