FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27713583
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACG59050.0132.83976
ACGTCGG138550.0128.297239
CCGATCA218900.0127.9120253
GTCGACG39450.0127.163498
CGACACG90700.0126.297485
CGTCGCG62550.0124.711226
CGCGTTC97800.0124.541855
CGGACGT87300.0124.3779141
TTGATCG221950.0123.855849
TCCCGAT204150.0123.567581
GACGGCT206350.0123.558611
CGCGCAA54850.0123.324654
GCCGATC155400.0122.981352
GCTACCG176200.0122.824022
TCGTCGC63700.0122.580425
GGCCGAT161650.0122.514841
ACGTTCG55700.0121.4358756
CACGTCG146700.0121.169598
CTCGCGT103850.0121.056323
CGCGGTA52900.0120.424031
Produced by FastQC (version 0.11.5)