FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27713583
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC116950.0121.983725
CACGACG118600.0120.5311364
CGACGCT119400.0119.0471346
ACACGAC138800.0103.929933
CTACACG141450.0103.6656651
GACGCTC138300.0102.935267
TACACGA145000.0100.190082
ACGCTCT152000.093.849488
CGCTCTT173500.082.053059
TCCGATC145100.019.31295415-19
TTCCGAT157450.018.29589310-14
CGTCGAC8500.017.9092483
CTTCCGA166800.017.4702210-14
TCTTCCG174750.016.68373310-14
GTCGACG9650.016.5261764
CCGATCT174150.016.32444415-19
CGCCGTA309650.015.75990660-64
TCGCCGT313250.015.48622160-64
GCCGTAT316150.015.37626860-64
CGCGAAA12150.015.0708441
Produced by FastQC (version 0.11.5)