FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28847706
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGT157550.0136.169561
CGCGGTA149400.0135.050321
CGCGATA105750.0132.734282
CTCGTAC249550.0132.315921
ACGCGTA72750.0132.150442
GTACGAC137450.0129.534019
TCCGCGA72700.0129.331928
GCTCGAC178950.0129.2462
GTTCGCG89950.0129.029898
CGTAGCG76600.0127.747111
TCGCGAG162000.0127.699471
CGTTCGC91200.0127.340877
ACGGGTC224100.0127.219191
TCCGTCG65150.0126.7556844
GCGCAAC173650.0126.612171
ACCGTAA255000.0126.454671
ACGCGTC90100.0126.257232
AATCGCG97100.0125.8000649
TAACGCG69300.0125.73949
CGCGTAC62000.0125.711475
Produced by FastQC (version 0.11.5)