FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28847706
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC133400.0120.749975
CACGACG135400.0119.073454
CGACGCT134600.0118.635136
CTACACG157150.0104.217491
ACACGAC155550.0104.1146853
GACGCTC154850.0103.4018557
TACACGA163350.099.457982
ACGCTCT170700.094.014818
CGCTCTT196750.081.861979
TCCGATC169250.018.70699315-19
TTCCGAT180650.018.04867210-14
CTTCCGA185000.017.78101210-14
TCTTCCG197000.016.71998410-14
CGCCGTA322500.016.67391460-64
TATCGCG10500.016.568189
TCGCCGT325250.016.4705360-64
CGCGTAC9450.016.2768611
GCCGTAT331150.016.23399460-64
CCGATCT197300.016.17969315-19
CGTATCA331700.016.14590365-69
Produced by FastQC (version 0.11.5)