FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22844444
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACG144150.0126.124134
ACGACGC145100.0125.048565
CGACGCT145700.0123.87386
CTACACG165550.0111.202541
ACACGAC164700.0110.915493
GACGCTC168000.0107.9056247
TACACGA173150.0105.631242
ACGCTCT177550.0101.8577968
CGCTCTT193900.093.1944059
CGTGCGA14300.026.6390481
TTACGCG7750.020.5759856
TACGCGA7550.020.1609957
CTTCCGA195400.018.94151510-14
TCCGATC188300.018.82380915-19
TTCCGAT194750.018.73674810-14
TCTTCCG207450.017.72945810-14
CGAACTA26600.017.167399
CCGATCT231750.015.44472915-19
CGATCTA58450.013.34371415-19
GACCGAT21300.012.5923684
Produced by FastQC (version 0.11.5)