FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27486902
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTA97350.0132.009451
TAGCGCG68750.0130.63937
GTCGCGT102350.0130.318248
GGCCGAT262850.0129.125751
GTCGTAC188850.0128.895849
CCGATAG170150.0128.308653
CGGTCGC103200.0127.5590746
GCCGATA170900.0127.533432
GGTCGCG105000.0125.510427
GCGGTCG105150.0125.400325
AGGTCCG189550.0124.9906161
CGGATCG83450.0124.913044
AATCGGT196450.0124.4704361
CTTCGAC183400.0123.912026
CGTCGAC54950.0123.080486
ATCCGCG47750.0122.9665157
TCGACAA287800.0122.0332648
GATCGAT161800.0121.352751
CTCGTCG56550.0121.264524
TTTGCGC139150.0120.354971
Produced by FastQC (version 0.11.5)