FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27486902
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC188550.0126.028885
CACGACG190600.0124.7494354
CGACGCT193450.0122.461456
ACACGAC205850.0115.859773
CTACACG207500.0115.522061
GACGCTC213200.0111.1082157
TACACGA216600.0110.7773062
ACGCTCT226600.0104.3928768
CGCTCTT250700.094.128569
TTCCGAT242000.019.9703410-14
TCCGATC234600.019.9454915-19
CTTCCGA244800.019.89592410-14
TCTTCCG256300.018.87308910-14
CCGATCT268200.017.69001815-19
CGATCTA77700.014.50019115-19
CGCCGTA368100.014.13891560-64
CGTATCA369450.013.9662165-69
GCCGTAT372750.013.89641760-64
TCGCCGT373750.013.86699360-64
GAACGCG19950.013.4457059
Produced by FastQC (version 0.11.5)