FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25847814
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG53000.0133.352689
AACGTCG66900.0129.592214
CGAATCG73550.0129.012224
CTCGTAC154000.0128.703581
GCGGTCG89950.0127.813255
CGCTCGA84400.0127.4574665
CGTACGT83200.0127.403913
CGCGTTT125850.0126.992711
AACCGCG68850.0125.833491
CGAAGCG98600.0125.716479
GCGACGT58700.0125.219759
CGTCCGA75950.0124.648896
CGGGTCG88200.0124.6204452
AGCGCGA93850.0124.432628
GTTACGC90350.0123.155338
CCGCTCG88600.0122.6427154
ACGATCG43650.0122.060994
TTGATCG203750.0121.461579
TTCGGTC144600.0121.396381
ATCGACG42350.0121.186117
Produced by FastQC (version 0.11.5)