FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41308397
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTA131950.0124.321351
CGATTCG97300.0123.372934
CGATCGA115250.0120.573975
GACGCGT90900.0119.344471
CGTATCG57450.0118.7331544
GACACGC256950.0116.531571
CGGACGT95450.0116.489171
CGTCGAC59500.0115.738257
CGCGATT120500.0114.565342
GACGGCT227250.0114.326221
TCGTTCG75850.0113.43988
TCCGCGA69950.0112.334178
CCGATCG68400.0110.323244
ACGATAC144150.0107.8661351
ACCGTAA201450.0107.812141
GCGACGT114450.0107.31763
CAGCGAC261850.0106.6177141
TACTCGC159100.0106.368511
TAAACCG127100.0106.231581
TACGGAT175150.0106.137411
Produced by FastQC (version 0.11.5)