FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32543946
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCGA158100.0127.55498
GTTCGGG268950.0127.129531
TCCGCGA89950.0127.087378
CGCGGTA129500.0127.017431
ACGGTCG65400.0126.135234
ACGCGAT159550.0126.0776449
CATATCG202400.0124.20544
ATCCGCG79300.0123.953387
TTACGCG40900.0122.82369
GTCGACG51000.0121.667757
GTCCGAC92650.0119.2371147
CTTACCG133900.0118.748821
TCGACGA60350.0118.432688
TTCGGGC193950.0118.355862
CGGTTAA197350.0117.835771
GGCGACT157800.0117.719461
TGCGATA186350.0116.023742
CGTAACG51650.0115.3648157
TAACCGA161950.0115.16779
TCGTTCG54950.0114.768698
Produced by FastQC (version 0.11.5)