FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32543946
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC115300.0117.697215
CGACGCT116000.0115.7990656
CACGACG118000.0115.065764
ACACGAC139800.097.952413
GACGCTC139600.095.902917
CTACACG145900.093.965861
TACACGA147300.093.432642
ACGCTCT156100.085.8242658
CGCTCTT181650.073.794599
ACGCGAC16950.021.8175322
CGCGACT19200.020.7659513
GACGCGA20700.018.4267621
TCCGATC146050.018.0492915-19
CGCCGTA439350.017.89099760-64
TTCCGAT155950.017.59136210-14
TCGCCGT445750.017.49101860-64
GCCGTAT453050.017.32438360-64
GTCGCCG459100.017.10555560-64
CTTCCGA162000.017.04180710-14
CGTATCA455100.017.0273765-69
Produced by FastQC (version 0.11.5)