FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25758836
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGA94700.0134.415189
TACTCGC196650.0133.763641
CGATTCG93800.0131.764334
CGCGTTA99550.0131.394182
GTACGCG101600.0130.566318
CGGACGT136300.0130.341061
GTCGCGT106750.0130.03938
TCGTTCG100650.0129.782098
GATCGTA208400.0128.466981
CGGTCCG89850.0128.03695
CGTCGAC56150.0127.291956
CCGATAG172200.0127.119113
GGCTCGA306350.0127.1025161
CGATTGA246450.0126.639211
TCGTACG65350.0126.565066
CGGTACG57400.0126.287936
AACGTCG57450.0125.169344
GGCCGAT179050.0124.944741
TAGGTCG154250.0124.4888847
ACGTCGA57800.0124.160565
Produced by FastQC (version 0.11.5)