FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25758836
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC193900.0126.103295
CACGACG194650.0125.543164
CGACGCT197000.0123.897896
CTACACG214450.0115.9990841
ACACGAC214250.0114.8026053
GACGCTC216750.0112.398637
TACACGA223600.0110.7496952
ACGCTCT228300.0106.820148
CGCTCTT248000.098.27969
TTCCGAT237150.020.74527510-14
TCCGATC232800.020.51023515-19
CTTCCGA242050.020.4511110-14
TCTTCCG256900.019.3592510-14
CCGATCT267450.018.12948815-19
CGCCGTA332250.016.08331160-64
GCCGTAT335550.015.82576960-64
CGTATCA335800.015.77137265-69
TCGCCGT338900.015.61860-64
TATCGCG10500.015.3704361
CCGTATC337150.015.15780965-69
Produced by FastQC (version 0.11.5)