FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27333800
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC166400.0125.638565
CACGACG168750.0123.803014
CGACGCT168300.0123.143156
ACACGAC186200.0112.706743
CTACACG188000.0112.296151
GACGCTC189800.0108.8033457
TACACGA194850.0108.445312
ACGCTCT202950.0101.5168848
CGCTCTT224650.091.615759
TCCGATC209250.019.52738815-19
CTTCCGA217400.019.48842810-14
TTCCGAT217450.019.35725410-14
TCTTCCG232100.018.18541310-14
CGACCGT11250.017.3963157
CCGATCT247000.016.74840515-19
TACGCGA9659.094947E-1213.5259212
CGATCTA69150.013.33624615-19
CGCCGTA290400.012.94454760-64
GTACGCG10853.6379788E-1212.8456411
TCGCCGT295100.012.60082860-64
Produced by FastQC (version 0.11.5)