FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25431465
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA75150.0129.572042
CGTACCG50800.0128.424857
CCGATCG55850.0128.104974
ACGTACG66700.0126.175976
CGGCTAG205850.0126.103491
CGTACGT66950.0125.9213647
TCGCGAG111550.0125.298431
TAGTCGA156500.0124.458715
TAGGTCG114150.0123.703889
CTTACCG146750.0123.2890241
TATCGCG53700.0122.2993556
TAACGCG46300.0120.892832
TCTACGG133050.0118.281078
GATCCGA102050.0117.700899
GATCGAT132400.0117.382291
TCGTCGC46600.0117.288586
CGCAACT119350.0116.578032
GGAGCGA194200.0116.04919
GACGCGT42250.0115.7515261
ATCGTTA168150.0115.7491157
Produced by FastQC (version 0.11.5)