FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26685132
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG190650.0136.155829
TAGCGCG119450.0135.388617
ACGCCGA149450.0135.142651
GTCCGCG158800.0134.386617
GACGCGT137500.0134.341451
CGCCGAA145100.0133.617832
TACTCGC165250.0133.191571
TCGCGCA128950.0133.090583
TGTCGCG111000.0132.634237
GCGTTCG126600.0131.635794
TCCGCGG162950.0131.497888
CGGACGT114350.0131.174271
AATCGGT254550.0130.833241
CGTAACG114800.0130.201347
AGCGCGT124550.0129.495598
CGGGCTA254700.0129.097782
GCGCGTC125350.0128.726979
TAGTCCG166450.0128.645725
ACGCGTT139750.0128.252872
CGACACG93650.0127.948085