FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26685132
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC158700.0124.3807145
CACGACG160600.0122.864314
CGACGCT160050.0122.787846
CTACACG181100.0110.210881
GACGCTC180250.0109.138277
TACACGA186000.0107.013942
ACACGAC184800.0106.971073
ACGCTCT194000.0101.11758
CGCTCTT214000.091.705099
TCCGATC198650.019.430715-19
TTCCGAT210550.018.7248310-14
CTTCCGA213900.018.6078210-14
CGCGACA16150.018.403254
CGATACG9800.017.752864
TCTTCCG226050.017.66539610-14
CCGATCT231600.016.81653215-19
ACGCGAC11000.015.1571773
CGATCTA75900.014.67196915-19
CGGCGAT14000.014.6704821
TAGCGCG11250.014.3444711
Produced by FastQC (version 0.11.5)