FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27653330
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCG130200.0135.346824
ACGTCGT117000.0132.988883
TCGTACG91050.0132.349582
CGGACGT133800.0131.891191
CACGTCG177800.0131.687358
CGTAACG71500.0130.683337
GCGCGTA63150.0130.515234
CGATCGG81400.0130.399771
CGCGCAA102950.0129.135934
CGTACGT92450.0128.14943
TTGCGCG68000.0127.8444062
TTCCGCG89800.0127.219357
GTGCGAA215250.0126.995028
AACGCCG103400.0126.891169
ATCGCGA69450.0126.741232
TGGTTCG173300.0125.528274
GTTCGCG74850.0125.2218256
CATCGGG213450.0124.8348851
TCGCACG74100.0124.630785
TAAACCG176550.0124.260441
Produced by FastQC (version 0.11.5)