FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27653330
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT112550.0119.026316
ACGACGC113800.0118.419825
CACGACG116050.0115.998954
GACGCTC130050.0102.7783667
ACACGAC133100.0101.4118963
CTACACG138450.098.3284761
TACACGA139050.097.777762
ACGCTCT144450.092.545618
CGCTCTT169450.078.423019
TCCGATC139500.018.72020115-19
CTTCCGA151450.018.08532310-14
TTCCGAT150200.017.92691610-14
CGACGTA11400.016.5345029
TCTTCCG167350.016.349710-14
CCGATCT168350.015.75330215-19
CGCCGTA282350.015.46001660-64
TCGCCGT285800.015.07049260-64
GCCGTAT291550.014.95227860-64
CGTATCA294500.014.60626465-69
GTCGCCG298150.014.57752560-64
Produced by FastQC (version 0.11.5)