FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28133060
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC120500.0122.423265
CACGACG121800.0121.3550954
CGACGCT122100.0119.750146
GACGCTC137400.0106.827737
CTACACG145300.0103.535961
ACACGAC146600.0101.567473
TACACGA151400.098.808662
ACGCTCT154750.095.004278
CGCTCTT181200.081.098539
TCCGATC147350.019.4642915-19
TTCCGAT160350.018.44666710-14
CTTCCGA169500.017.60485310-14
TCTTCCG177350.016.84196310-14
CGTCGAC9100.016.7288483
CCGATCT176700.016.39537415-19
CGCCGTA317100.015.56235960-64
TCGCCGT322950.015.17721360-64
GCCGTAT328400.015.04452860-64
CGTATCA330850.014.87266965-69
GTCGCCG334250.014.78555860-64
Produced by FastQC (version 0.11.5)