FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29254044
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC135050.0122.331385
CGACGCT137350.0119.6491246
CACGACG139350.0118.868664
ACACGAC159100.0104.6140063
CTACACG161150.0104.5389941
GACGCTC159950.0102.554577
TACACGA165700.0100.9990542
ACGCTCT180400.091.180448
CGCTCTT201700.081.445569
TCCGATC172750.018.68407415-19
TTCCGAT180300.018.40793410-14
CTTCCGA189600.017.61972410-14
CGGTACG8350.017.3642859
CGACGTC15600.017.193486
TCTTCCG201450.016.61925910-14
CGCCGTA327300.016.43740560-64
TCGCCGT329650.016.20148760-64
CCGATCT202850.016.11892315-19
GCCGTAT335800.015.99974360-64
CGTATCA338000.015.797708565-69
Produced by FastQC (version 0.11.5)