FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24838603
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACG293250.0131.366614
ACGACGC294500.0130.522745
CGACGCT294800.0129.628436
CTACACG305500.0127.178211
ACACGAC311400.0124.151573
GACGCTC315800.0120.985167
TACACGA327200.0118.488982
ACGCTCT331350.0115.241558
CGCTCTT357450.0106.888219
TTCCGAT360450.021.7036210-14
CTTCCGA367650.021.4758610-14
TCCGATC357000.021.32862915-19
TCTTCCG373700.020.96514710-14
CGCGCAT13950.019.972821
CCGATCT395750.019.52597415-19
CGACGGT11750.017.4723031
CGATCTA102000.017.28382515-19
CGATCTG124050.015.73094115-19
GCTCTTC508900.015.45232310-14
CGATCTT150700.012.9586815-19
Produced by FastQC (version 0.11.5)