FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25844936
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTA90200.0130.679761
GGCCGAT244400.0130.02581
TAGCGCG63050.0128.547937
CCGATAG159200.0128.247573
GCCGATA160000.0128.1042
GTCGCGT95400.0126.980328
CGGTCGC94750.0126.5504766
GTCGTAC168100.0126.532119
CTTCGAC175400.0124.117576
AATCGGT184850.0123.901281
AGGTCCG179650.0123.424931
ACGCGTA55400.0123.126584
TCGACAA269650.0122.810098
CGTCGAC52750.0122.725986
GGTCGCG97600.0122.706797
GCGGTCG98100.0122.148895
GATCGTA158900.0122.088121
TTTGCGC135150.0120.6450351
CGGATCG81950.0120.604044
CTCGTCG54800.0120.502084
Produced by FastQC (version 0.11.5)