FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25844936
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC187000.0126.33415
CACGACG189500.0124.7654
CGACGCT189900.0123.891696
ACACGAC201750.0117.3150253
CTACACG206450.0115.533941
GACGCTC208450.0112.761197
TACACGA216800.0109.476512
ACGCTCT221400.0106.0341958
CGCTCTT244400.096.2938469
CTTCCGA243150.020.02095810-14
TTCCGAT241800.019.96476410-14
TCCGATC234900.019.78673715-19
TCTTCCG254800.019.04293410-14
TACGACG10500.018.0106855
CCGATCT268650.017.60856415-19
TACGCGT9350.015.5611977
CGATCTA77450.014.71278215-19
CGCCGTA347950.014.35066360-64
TATCGCG8802.1827873E-1114.1603231
CGTATCA348050.014.12618565-69
Produced by FastQC (version 0.11.5)