FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22661437
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGAA125450.0137.859392
TTGCGCG134300.0136.884082
TTACGCG83450.0136.824699
CGCGATA122150.0136.650342
TACGTCG67750.0136.529132
GTACGCG103400.0136.437128
TACGCGA99500.0135.810419
CGGGTCG265300.0135.0744
AACCGCG150200.0135.07391
ACGCCGA131550.0134.917821
ACGATCG144200.0134.672474
CGTACGT111350.0134.378467
GCCGCGT176350.0133.889073
ACGTACG111650.0133.692756
ACGTCCG145900.0133.604425
CGCAACA356900.0133.410522
CGTTACG85250.0133.25547
TCGGTCG122750.0133.197984
CGCGTGA174000.0132.567235
GTCGCGA78450.0132.145787
Produced by FastQC (version 0.11.5)