FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24500145
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTA222350.0139.396621
ATTCGCG108950.0136.755892
CGGTTCG116050.0135.139924
TAACGCG111800.0133.70689
CCGTACT211600.0131.195851
TATAGCG198500.0129.205475
CGGTTAA257000.0128.640761
TACTCGC128150.0127.881321
GTACGTA262050.0127.833091
AGGCGAC217750.0127.710648
AACCGCG68200.0126.943281
GGCTACG176850.0126.706999
CGCTCCG112300.0125.939265
CGGTCGT73550.0125.77293
GGTAACG195800.0125.477417
CCGGTCG69300.0125.21165
CGGTATC132800.0124.498853
AATCGGT233950.0123.8730551
CAAGCCG172500.0123.72449
GCGGTAT135550.0123.57882
Produced by FastQC (version 0.11.5)