FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37643561
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT94300.0110.7686546
ACGACGC98500.0106.907465
CACGACG99800.0105.615864
GACGCTC120100.087.699677
CTACACG129200.082.887661
ACACGAC128350.082.406863
TACACGA129950.081.599212
ACGCTCT137850.075.615688
CGCTCTT164800.063.5595139
CGCCGTA758900.020.34471360-64
TCGCCGT767400.020.01737460-64
GCCGTAT769250.019.98053760-64
CGTATCA778450.019.67961765-69
GTCGCCG787950.019.56337760-64
TGGTCGC812950.018.99372355-59
CCGTATC774900.018.92428665-69
GTGGTCG834950.018.60609655-59
GGTCGCC807350.018.2961660-64
CCGATCT154000.017.9441451
TCTCGGT881500.017.76024650-54
Produced by FastQC (version 0.11.5)