FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24164575
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG50950.0134.320929
AACGTCG60600.0130.166174
CGAATCG73100.0129.151984
CGCGTTT117100.0128.754651
CTCGTAC145250.0126.661961
AACCGCG62250.0126.6117251
CGTACGT77500.0126.477563
GCGGTCG85150.0126.42335
CGCTCGA80900.0125.896345
GCGACGT54900.0125.713099
GTTACGC89050.0125.128388
AGCGCGA87750.0124.751488
CGAAGCG95100.0124.6387569
CGTCCGA72350.0122.6475146
CGAGTCG48100.0122.526715
CGGGTCG84400.0122.330822
GTCCGCA153850.0122.232968
CCGCTCG84550.0121.789534
TTGATCG190500.0121.702459
GTCCGAC73000.0121.654777
Produced by FastQC (version 0.11.5)