FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30225246
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCGA154800.0128.461338
ACGCGAT154600.0128.017919
TCCGCGA81900.0127.6435858
CGCGGTA115050.0125.602761
ACGGTCG64600.0125.146744
CATATCG190850.0124.3433154
GTTCGGG253150.0124.285561
ATCCGCG73500.0123.096257
TTACGCG38450.0122.178919
GTCGACG43650.0122.0732967
CTTACCG130550.0119.5229951
CGGTTAA189550.0118.5128561
GTCCGAC88750.0117.628277
TCGACGA54050.0117.496798
TCGTCCG55150.0117.3875666
CGCTATC124550.0117.077221
TTCGGGC173650.0116.3690952
TAACCGA143250.0116.34889
GGCGACT139150.0115.964781
CCGATCA130850.0115.922453
Produced by FastQC (version 0.11.5)