FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30225246
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC106450.0116.89495
CGACGCT106200.0116.09546
CACGACG109150.0114.156774
GACGCTC127450.097.308987
ACACGAC129750.096.515883
CTACACG132800.094.7024841
TACACGA134200.093.4550552
ACGCTCT145000.085.280418
CGCTCTT166850.073.9818959
CGCCGTA423050.018.53557460-64
TCGCCGT430850.018.15291460-64
GCCGTAT435250.018.01935460-64
TCCGATC135150.017.85037215-19
CGTATCA437400.017.77214465-69
GTCGCCG450750.017.518760-64
TTCCGAT144150.017.34869410-14
CCGTATC435900.017.18820265-69
CTTCCGA148650.016.97964110-14
TGGTCGC467150.016.97807155-59
GTGGTCG485350.016.37127955-59
Produced by FastQC (version 0.11.5)