FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25573800
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG145200.0136.368411
GACGTTA378150.0135.527471
CCTTACG297700.0133.83351
GTCGCGT120250.0133.29758
GGACGTC267650.0132.525561
TAGGTCG221850.0132.468865
CGCGGTA108450.0132.307531
ACGGTCG89500.0132.243424
TTTGCGC202200.0131.133871
CGCGTTT171900.0131.108861
TCGTCCG82200.0129.999956
TTCCGCG106250.0129.641197
CTTACGG283600.0129.522112
CGGACGT104900.0129.479641
GCGCAAC171550.0129.03641
GTTCGGG287250.0128.310421
GACGTCG73350.0127.499359
TCGCGTA123850.0126.203429
TTATCGG182600.0126.1660845
GTCGCCG119200.0126.0786069
Produced by FastQC (version 0.11.5)