FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25044187
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG177900.0136.922919
CGCCGAA139100.0135.621252
TAGCGCG109750.0135.017217
ACGCCGA147250.0134.476471
GACGCGT128050.0134.12021
GCGTTCG117000.0132.688724
GTCCGCG152250.0132.325657
TGTCGCG97700.0131.931957
TACTCGC155800.0131.921941
TCGCGCA120050.0131.678943
AATCGGT236800.0129.640121
TCCGCGG156150.0129.438548
CGGGCTA242050.0129.056842
CGGACGT109550.0128.920041
CGTAACG112500.0128.816967
CGTATCG54750.0128.440037
ACGCGTT130500.0128.032972
GTAACGC192800.0127.544468
GCGCGTC116400.0127.428179
CGCGTTC129100.0127.116613
Produced by FastQC (version 0.11.5)