FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25044187
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC164050.0125.7607355
CGACGCT166800.0123.5862666
CACGACG167000.0123.5453954
CTACACG182800.0114.30681
GACGCTC186300.0110.76727
ACACGAC187900.0110.260163
TACACGA188950.0110.075752
ACGCTCT196500.0104.795348
CGCTCTT216450.095.036029
TCCGATC203700.019.85033815-19
CTTCCGA217900.019.26563510-14
TTCCGAT216250.019.19127510-14
TCTTCCG230950.018.23354510-14
CGATACG9000.017.7709564
CCGATCT232250.017.6411515-19
CGACGGT14400.015.2689721
CGATCTA74500.015.12081415-19
CGCCGTA286500.013.88225860-64
CGCGTTA12600.013.8326772
GCCGTAT292200.013.59657460-64
Produced by FastQC (version 0.11.5)