FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25940773
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCG125550.0135.48034
ACGTCGT113050.0134.024733
TCGTACG84550.0132.404312
CGTACGT84150.0131.308733
GTTCGCG68600.0130.725046
CACGTCG168750.0130.643148
CGTAACG66650.0129.328937
CGGACGT127000.0129.211581
CGCGCAA97900.0128.158554
AACGCCG93250.0127.655149
GCGCGTA57100.0127.213524
CGATCGG72950.0126.82641
TTCCGCG84100.0125.9413157
ATCGCGA64750.0125.016252
GTGCGAA204050.0124.847298
TCGCACG70500.0124.830565
TGGTTCG169800.0124.790194
TCGCGCA102450.0124.4358753
CATCGGG199350.0123.749831
GGCGACT151850.0123.586741
Produced by FastQC (version 0.11.5)