FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25940773
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC110000.0119.150815
CGACGCT109700.0119.039956
CACGACG111000.0118.3653954
GACGCTC128000.0102.247917
ACACGAC130650.0101.037483
CTACACG131850.0100.346491
TACACGA133850.098.910712
ACGCTCT140700.092.4499368
CGCTCTT163450.079.849239
TCCGATC137100.018.6855115-19
CTTCCGA148450.018.00923310-14
TTCCGAT146300.017.98635110-14
CGACGTA11200.017.5379569
TCTTCCG164550.016.32650410-14
CGCCGTA266950.016.2019860-64
CCGATCT162750.015.89204215-19
TCGCCGT273900.015.74853460-64
GCCGTAT276850.015.50742660-64
CGTATCA279550.015.29573265-69
GTCGCCG288500.015.07715660-64
Produced by FastQC (version 0.11.5)