FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26387774
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGG139750.0128.960759
CCGATCA207800.0128.865173
CGGTACG56100.0128.449316
CGTCGCG58000.0126.491336
CGACACG85700.0126.4589845
GCCGATC147900.0125.284622
GTCGACG35950.0124.017938
CACGTCG145900.0123.624158
GGCCGAT154850.0123.622331
TTGATCG212700.0123.2108849
TCGTCGC59500.0123.053445
GACGGCT206950.0122.86341
CGCGTTC97500.0122.8272555
TCCCGAT198750.0122.791681
GCTACCG171750.0122.3875052
CTCGCGT100750.0122.083953
CGGACGT83900.0121.432041
CGCGGTA53650.0121.068211
CATAGCG165450.0120.4473655
CCGGTAG131450.0119.9231341
Produced by FastQC (version 0.11.5)