FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27416770
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC136700.0123.643495
CGACGCT139800.0120.715766
CACGACG140450.0120.092024
ACACGAC157750.0107.1227343
GACGCTC159850.0105.75627
CTACACG160900.0105.7357941
TACACGA165050.0102.436122
ACGCTCT176150.096.0939568
CGCTCTT194700.086.864079
CGACGTC14800.020.1514026
GTCGTAC20050.019.197612
TCCGATC174400.018.87069115-19
TTCCGAT181500.018.58137910-14
CGCGAGT25400.018.1806661
CTTCCGA188400.018.03943810-14
CGCCGTA322550.016.97268960-64
TCTTCCG202050.016.94996310-14
TCGCCGT323850.016.82399660-64
GCCGTAT330100.016.51423660-64
CGTATCA328050.016.49406465-69
Produced by FastQC (version 0.11.5)