FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21675117
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC150850.0124.0158845
CACGACG151200.0123.629754
CGACGCT151400.0123.057696
ACACGAC164800.0113.6453863
CTACACG165900.0113.445621
GACGCTC170600.0109.207797
TACACGA175450.0107.4909742
ACGCTCT182300.0101.91958
CGCTCTT195500.094.9263469
TCCGATC188650.019.5046115-19
TTCCGAT194000.019.46291510-14
CTTCCGA198550.019.1557110-14
TCTTCCG204300.018.63077410-14
GACCGAT21500.017.5848064
CGTGCGA15250.016.3462181
CCGATCT235850.015.79791315-19
GCGCGTT15650.015.7767672
CGAACTA25500.014.8349549
ACCGATC26650.014.7383295
CGATCTA62050.013.87864715-19
Produced by FastQC (version 0.11.5)