FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005403543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80041321
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC541250.0126.108565
CACGACG545800.0125.100634
CGACGCT548650.0123.919136
ACACGAC593450.0115.262373
CTACACG599650.0114.849261
GACGCTC612100.0111.1332247
TACACGA630750.0108.711182
ACGCTCT648150.0104.673758
CGCTCTT715700.094.672769
TCCGATC673000.019.82775315-19
CTTCCGA701850.019.77685410-14
TTCCGAT695900.019.76884310-14
TCTTCCG738650.018.81707810-14
CCGATCT776250.017.43139815-19
CGATCTA221950.014.72508715-19
CGCCGTA1076450.014.24534260-64
CGTATCA1087300.013.9462465-69
GCCGTAT1098100.013.90638660-64
TCGCCGT1097400.013.849195560-64
CCGTATC1093000.013.3826765-69
Produced by FastQC (version 0.11.5)