FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005403547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98784853
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC723850.0126.125845
CACGACG731900.0124.880884
CGACGCT729000.0124.598546
ACACGAC791750.0115.678153
CTACACG801400.0114.741341
GACGCTC801400.0113.342197
TACACGA821950.0111.437412
ACGCTCT845050.0107.1029368
CGCTCTT931200.097.139819
TCCGATC862700.020.58223315-19
CTTCCGA894750.020.53997410-14
TTCCGAT884850.020.53709610-14
TCTTCCG962200.019.21766910-14
CCGATCT985100.018.25006315-19
CGCCGTA1274350.015.93115860-64
CGTATCA1299700.015.51820865-69
GCCGTAT1307300.015.47638360-64
TCGCCGT1307300.015.43534760-64
CGATCTA268600.015.40117315-19
CCGTATC1303550.014.92066465-69
Produced by FastQC (version 0.11.5)