FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12393909
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC74250.0123.319625
CGACGCT74350.0122.570196
CACGACG76350.0119.933544
CTACACG83000.0111.111651
TACACGA85000.0107.898262
ACACGAC84750.0107.8739243
GACGCTC84500.0107.847717
ACGCTCT89800.0101.3214658
CGCTCTT100900.090.31889
CTTCCGA94700.019.62862410-14
TCCGATC90600.019.62090315-19
TTCCGAT96200.019.03619210-14
CGATACG3852.3900066E-718.8315324
TCTTCCG102550.018.18264810-14
CCGATCT103900.017.34651815-19
ACGCGAC5554.0110535E-815.6757873
CGCGCTA5304.061476E-715.0474594
CGATCTA32100.015.04166415-19
CGACGGT5154.6635014E-614.1671791
CGCGACA8151.7698767E-913.3438144
Produced by FastQC (version 0.11.5)