FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005403552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60735522
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCG290450.0134.030384
ACGTCGT270350.0133.296843
CACGTCG398100.0133.04158
CGTAACG158550.0132.184787
CGGACGT305300.0131.299681
TCGTACG193600.0131.245732
CGTACGT192700.0130.241043
CGCGCAA224650.0130.111154
GCGCGTA133100.0129.35584
CGATCGG179750.0128.472781
TTGCGCG142650.0127.861962
GTTCGCG161000.0127.831876
ATCGCGA145650.0126.771312
AACGCCG220400.0126.567049
GTGCGAA484250.0126.319258
TTCCGCG194750.0125.854097
TCGCGCA235700.0125.580523
TCGCACG163200.0124.998875
CATCGGG460050.0124.943841
GGCGACT349550.0124.731171
Produced by FastQC (version 0.11.5)