FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005403557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67120033
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC311300.0120.7525
CACGACG315950.0119.184894
CGACGCT315000.0118.3900156
ACACGAC358600.0105.211063
CTACACG363700.0104.5794751
GACGCTC361650.0103.239237
TACACGA374000.0101.189372
ACGCTCT395450.093.884678
CGCTCTT449550.082.666989
TCCGATC391250.018.54486315-19
TTCCGAT413300.018.22532310-14
CTTCCGA427150.017.88896610-14
TCTTCCG454400.016.84489810-14
GTCGTAC47450.016.6542952
CCGATCT454350.016.28848615-19
CGCCGTA775950.016.09405160-64
CGACGTC36450.015.911726
GCCGTAT788400.015.839960-64
TCGCCGT782300.015.83923560-64
CGTATCA791150.015.60190565-69
Produced by FastQC (version 0.11.5)