FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005403558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174634509
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG481750.0127.368471
CGGACGT526400.0122.590721
GCGCAAC797050.0122.555481
TACGGGC1143650.0120.7859951
TCGGTCG348800.0120.773842
ACGCCGA630700.0120.376481
CTTACCG701700.0118.908871
TTACGCG419300.0118.717469
TTGCGCG272300.0116.980462
TCCGCGA298350.0116.162278
TTCGGTC829000.0115.999071
CGCCGAC450600.0115.41572
CGCGGTA313500.0114.7571951
CCTTACG682500.0114.612451
CGTTACG202500.0114.37167
GACGTTA781250.0114.232141
AACGTCG235800.0114.11225
TTTGCGC672300.0113.915461
GTTACGC449250.0111.335438
GTCGCCG373100.0111.3078849
Produced by FastQC (version 0.11.5)