FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005403562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171161551
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTA547400.0126.1275941
CGATTCG425050.0125.384974
CGATCGA450050.0121.927965
GACACGC1064600.0118.145941
CGTATCG240100.0118.039324
GACGCGT374750.0117.7902151
CGCGATT503600.0116.983712
CGGACGT389100.0116.119661
CGTCGAC253100.0114.9341357
GACGGCT953400.0114.1041641
CCGATCG272350.0113.943324
TCCGCGA291800.0113.8012548
ACGATAC592100.0112.344211
TCGTTCG324300.0110.577558
ACCGTAA829750.0109.083331
TACTCGC640700.0108.013981
GGCCGAT661400.0107.7122041
CAGCGAC1058500.0107.514281
TAAACCG548300.0106.019241
GCGACGT471850.0105.631023
Produced by FastQC (version 0.11.5)