FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005403563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171161551
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC504800.0116.121965
CGACGCT517250.0112.53236
CACGACG525800.0111.6771854
GACGCTC635750.091.545457
ACACGAC649500.090.943553
CTACACG662650.089.7695161
TACACGA671450.088.197962
ACGCTCT721950.080.785788
CGCTCTT855450.068.390369
CGCGAAA87550.025.6822051
TTCGCGA61100.024.9161782
TCGCGAC71050.021.8348243
CGCCGTA4233600.020.49726560-64
TCGCCGT4259150.020.28886260-64
TTTCGCG74850.020.2548311
GCCGTAT4268950.020.22632460-64
CGTATCA4297300.020.02806565-69
GTCGCCG4337650.019.8628660-64
TGGTCGC4469450.019.35146955-59
CCGTATC4293150.019.2527765-69
Produced by FastQC (version 0.11.5)