FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28076253
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC139600.0124.682945
CACGACG141550.0123.170154
CGACGCT141000.0123.088556
GACGCTC160150.0108.5512547
ACACGAC169900.0102.830973
CTACACG173000.0101.7775041
TACACGA175700.099.849742
ACGCTCT178350.097.433338
CGCTCTT198950.087.271859
CGGTACG9900.023.4323064
CGACGGT15300.022.9780521
TCCGATC175150.019.18792315-19
TTCCGAT184400.018.83928710-14
CTTCCGA187550.018.7006810-14
CGCGAAA13600.018.3106351
ACGGTAC32950.017.3808863
CGCCGTA390350.017.36045860-64
TCTTCCG202450.017.34582510-14
GCCGTAT397750.017.01560260-64
TCGCCGT395600.016.97980560-64
Produced by FastQC (version 0.11.5)