FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005403568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172654385
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG495850.0125.932451
GCGCAAC786100.0122.446611
CGGACGT511500.0121.950121
TACGGGC1116150.0120.659091
TTACGCG426950.0119.895869
ACGCCGA623400.0119.8717961
TCGGTCG351750.0119.562092
TTGCGCG272150.0118.3850252
CTTACCG690900.0117.114481
CGCCGAC445600.0116.359792
TCCGCGA298600.0116.351278
CCTTACG688200.0115.737691
TTCGGTC819450.0115.212821
AACGTCG238550.0114.555375
GACGTTA781450.0114.027111
CGCGGTA315150.0113.5122151
TTTGCGC664600.0113.46881
CGTTACG202500.0113.292777
GTTACGC457650.0112.32828
GTCGCCG371450.0110.704679
Produced by FastQC (version 0.11.5)